Next Generation Sequencing Technology can be used to generate gigabases of random short sequences from any DNA or RNA template. Our Centre adopted Illumina (formerly Solexa) technology early in 2008. We are able to provide both targeted and high volume sequencing applications utilising either the MiSeq or HiSeq platforms.
NGS can be used to study interactions and modifications to DNA. Methylation is a methylation is an epigenetic mark that has a crucial role in many biological processes. Methylated DNA immunoprecipitation-sequencing (MeDIP-seq) is a modification of Chromatin immunoprecipitation (ChIP)Seq whereby an antibody is used to precipitate a particular DNA feature, in this case methylated DNA. The products of this are then sequenced using Illumina Sequencing Chemistry.
Bisulphite NGS can be used to look at per base resolution of methylation patterns.
To discuss projects or for costings please contact Dr Charles Mein on 020 7882 2055 or by email c.a.mein@qmul.ac.uk.
Genome wide methylation at 450,000 cpG sites can also be assayed using the Illumina meth450 array and individual sites with pyro sequencing.