Next generation sequencing technology can be used to generate gigabases of random short sequences from any DNA or RNA template. Our Centre adopted Illumina (formerly Solexa) technology early in 2008. We are able to provide both targeted and high volume sequencing applications utilising either the MiSeq or HiSeq platforms.
We have a range of library preparation protocols that can be applied to different scales of experiment; Fluidigm access arrays for small, targeted analysis, through Agilent Sure Select for larger custom regions and Exome sequencing and a simple, no enrichment protocol for sequencing whole genomes. The table below gives some more detail, please contact the centre manager to discuss project design.
Our service includes all process steps from receiving DNA through to delivery of QCed FastQ formatted files.
Genotyping of microsatellite or Short Tandem Repeats (STR) has been used extensively for a variety of standard genetic approaches. Fluorescently labeled oligonucleotide primers are used to PCR amplify loci before electrophoresis and detection on the Applied Biosystems 3730xl DNA analyzer. Loci can be multiplexed on both size (up to 500bp) and by dye colour to allow up to 20 loci to be analysed simultaneously.
The Genome Centre offers a flexible range of services for microsatellite or STR genotyping, including assay design, sample amplification, electrophoresis and analysis. We are also able to support MLPA assays, either self-designed or purchased direct from MRC-Holland. Our quality assurance tool ensures genotyping and interpretation accuracy.
Discuss project plans and costings with our centre manager on 020 7882 2055 or email at c.a.mein@qmul.ac.uk.