Skip to main content
Blizard Institute - Faculty of Medicine and Dentistry
  1. Blizard Institute
  2. Research
  3. Core Facilities
  4. Flow Cytometry
  5. Uses of Flow Cytometry
  6. Cell Cycle Analysis
  7. m phase

m phase

Histone H3

Histones are highly basic proteins that complex with DNA to form chromatin. H3 histone Flow cytometry may be used to analyse cell cycle with propidium iodide (PI) giving peaks of G1 and G2m with S phase located between these peaks. Labelling histone H3 with a fluorescently conjugated monoclonal antibody and then staining PI allows the investigator to determine which G2m cells are in the m phase of the cell cycle by flow cytometry. 

  • H3 protocol BD Phopho Kit Cat No 558217
Back to top