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The William Harvey Research Institute - Faculty of Medicine and Dentistry

Professor Ahuva Nissim

Ahuva

Professor in Antibody and Therapeutic Engineering

Centre: Biochemical Pharmacology

Email: a.nissim@qmul.ac.uk
Telephone: +44(0) 20 7882 3990

Profile

Ahuva Nissim graduated in Molecular Immunology in 1992 from the Weizmann Institute of Science in Israel and was trained as a postdoctoral fellow at the MRC Centre for Protein Engineering in Cambridge until 1995. During this period she developed phage display semi-synthetic human antibody library, the so call ‘Nissim’ library which has been used worldwide. In November 2000 she was appointed as a Senior Lecturer at Queen Mary University of London. Her studies are interdisciplinary and involve translational research at the William Harvey Research Institute and with intensive worldwide collaborations. Her studies have been mostly focused on the mechanisms that lead to the formation of disease tissue-specific pathogenic proteins and the exploitation of identified pathogenic proteins to develop platform technologies for novel disease - and tissue-specific, diagnosis and targeted treatment.

Research

Group members

  • MD/PhD research fellows: Dr Rocky Strollo (with Prof Pozzilli as part of international collaboration with Campus Bio-Medico University of Rome)
  • Students: Chiara Vinci (with Prof Pozzilli), Louise Mary Topping (with Helena Azevedo); Anastasija Pikus (with Prof Leslie)
  • Research staff: Dr Michael Tranter; Dr Sagar Raturi, Dr Stella Man

Summary

The main research interest focuses on development of diseased tissue specific diagnosis and targeted immunotherapy to novel neoantigens produced specifically in diseased tissue. The role of oxidants in generation of neoantigen in the diseased tissues as a results of oxidative post-translational modification is investigated. Research is focused on arthropathies and type 1 diabetes but other autoimmune condition such as periodontal diseases have been studied. Hence, a specific targeted immunotherapy for the treatment of arthropathies has been developed. Ongoing research activities include the development of novel human antibody repertoires for engineering of new treatment modalities.

 

Publications

  • Esposito I, Kontra I, Giacomassi C et al. (publicationYear). Identification of autoantigens and their potential post-translational modification in EGPA and severe eosinophilic asthma. nameOfConference


  • Strollo R, Vinci C, Man YKS et al. (2023). Autoantibody and T cell responses to oxidative post-translationally modified insulin neoantigenic peptides in type 1 diabetes. nameOfConference


  • Gigout A, Harazin D, Topping LM et al. (2021). Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species. nameOfConference


  • Vinci C, Infantino M, Raturi S et al. (2020). Immunoglobulin A antibodies to oxidized collagen type II as a potential biomarker for the stratification of spondyloarthritis from rheumatoid arthritis. nameOfConference


    QMRO: qmroHref
  • Topping LM, Thomas BL, Rhys HI et al. (2020). Targeting Extracellular Vesicles to the Arthritic Joint Using a Damaged Cartilage-Specific Antibody. nameOfConference


  • Nissim A (2019). 016 Targeting combined treatment to arthritic joints by antibody specific to damaged cartilage. nameOfConference


    QMRO: qmroHref
  • Strollo R, Vinci C, Napoli N et al. (2019). Antibodies to oxidized insulin improve prediction of type 1 diabetes in children with positive standard islet autoantibodies. nameOfConference


    QMRO: qmroHref
  • Nissim A (2019). TARGETING COMBINED TREATMENT TO ARTHRITIC JOINTS BY ANTIBODY SPECIFIC TO DAMAGED CARTILAGE. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Topping LM, Vinci C, Rhys H et al. (2018). OP0260 Targeting neutrophil microvesicles to damaged cartilage using antibodies to post-translationally modified collagen ii. FRIDAY, 15 JUNE 2018


    QMRO: qmroHref
  • Smallwood MJ, Nissim A, Knight AR et al. (2018). Oxidative stress in autoimmune rheumatic diseases. nameOfConference


    QMRO: qmroHref
  • Strollo R, Vinci C, Napoli N et al. (2018). Antibodies to oxidised insulin improve prediction of type 1 diabetes in children with positive standard islet-autoantibodies. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Topping L, Rhys H, Norling L et al. (2017). FRI0011 Targeting neutrophil microvesicles to damaged cartilage using antibodies to post translationally modified collagen ii. Poster Presentations


    QMRO: qmroHref
  • Vinci C, Infantino M, Pozzilli P et al. (2017). FRI0423 Antibodies to type ii collagen: a novel tool for the spondyloarthritis diagnosis?. Poster Presentations


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  • Strollo R, Vinci C, Napoli N et al. (2017). Antibodies to post-translationally modified insulin as a novel biomarker for prediction of type 1 diabetes in children. nameOfConference


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  • Nissim A, Hughes C, Faurholm B et al. (2017). 06.09 Targeting therapeutic and regenerative biomedicine specifically to arthritic joints. Novel technologies


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  • Strollo R, Vinci C, Napoli N et al. (2017). Antibodies to Posttranslationally Modified Insulin for Prediction of Type 1 Diabetes in Children. nameOfConference

    DOI: doi

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  • Rizzo P, Pitocco D, Zaccardi F et al. (2017). Autoantibodies to post‐translationally modified type I and II collagen in Charcot neuroarthropathy in subjects with type 2 diabetes mellitus. nameOfConference


    QMRO: qmroHref
  • Hughes C, Nissim A (2016). Progress and clinical potential of antibody-targeted therapy for arthritic damage. nameOfConference


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  • Nissim A (publicationYear). COMBINATORIAL ANTIBODY PHAGE DISPLAY LIBRARIES CONSTRUCTED FROM B-CELLS INFILTRATING BRAIN TISSUE OF PEOPLE WITH PROGRESSIVE MULTIPLE SCLEROSIS. nameOfConference


    QMRO: qmroHref
  • Lim NH, Vincent TL, Nissim A (2016). Detection of early osteoarthritis by in vivo optical imaging of an antibody specific to type II collagen damaged by reactive oxidants. nameOfConference


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  • Lim NH, Vincent TL, Nissim A (2015). In vivo optical imaging of early osteoarthritis using an antibody specific to damaged arthritic cartilage. nameOfConference


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  • Strollo R, Vinci C, Arshad MH et al. (2015). Antibodies to post-translationally modified insulin in type 1 diabetes. nameOfConference


    QMRO: qmroHref
  • (2015). Cohen-Saidon C, Nechushtan H, Kahlon S, Livni N, Nissim A, Razin E. A novel strategy using single-chain antibody to show the importance of Bcl-2 in mast cell survival. Blood. 2003;102(7):2506-2512.. nameOfConference


    QMRO: qmroHref
  • Kuhle J, Gaiottino J, Leppert D et al. (2015). Serum neurofilament light chain is a biomarker of human spinal cord injury severity and outcome.. nameOfConference


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  • Strollo R, Vinci C, Arshad MH et al. (2015). Autoantibodies to posttranslationally modified insulin in type 1 diabetes. nameOfConference

    DOI: doi

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  • Hughes C, Sette A, Seed M et al. (2014). Targeting of viral interleukin-10 with an antibody fragment specific to damaged arthritic cartilage improves its therapeutic potency.. nameOfConference


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  • Burska AN, Hunt L, Boissinot M et al. (2014). Autoantibodies to Posttranslational Modifications in Rheumatoid Arthritis. nameOfConference


    QMRO: qmroHref
  • Ryan BJ, Nissim A, Winyard PG (2014). Oxidative post-translational modifications and their involvement in the pathogenesis of autoimmune diseases.. nameOfConference


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  • Kuhle J, Plattner K, Bestwick JP et al. (2013). A comparative study of CSF neurofilament light and heavy chain protein in MS.. nameOfConference


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  • Puentes F, Topping J, Kuhle J et al. (2014). Immune reactivity to neurofilament proteins in the clinical staging of amyotrophic lateral sclerosis. nameOfConference


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  • Strollo R, Ponchel F, Malmström V et al. (2013). Autoantibodies to posttranslationally modified type II collagen as potential biomarkers for rheumatoid arthritis.. nameOfConference


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  • Eggleton P, Nissim A, Ryan BJ et al. (2013). Detection and isolation of human serum autoantibodies that recognize oxidatively modified autoantigens.. nameOfConference


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  • Strollo R, Rizzo P, Spoletini M et al. (2013). HLA-dependent autoantibodies against post-translationally modified collagen type II in type 1 diabetes mellitus.. nameOfConference


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  • Maggiore C, Giovannoni G, Martin J et al. (2013). Construction of an antibody phage display library from B-cells infiltrating brain tissue of people with progressive multiple sclerosis. nameOfConference

    DOI: doi

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  • Gaiottino J, Norgren N, Dobson R et al. (2013). Increased neurofilament light chain blood levels in neurodegenerative neurological diseases.. nameOfConference


  • Man YKS, DiCara D, Chan N et al. (2013). Structural guided scaffold phage display libraries as a source of bio-therapeutics.. nameOfConference


  • Wythe SE, DiCara D, Taher TEI et al. (2013). Targeted delivery of cytokine therapy to rheumatoid tissue by a synovial targeting peptide.. nameOfConference


    QMRO: qmroHref
  • Rizzo P, Pitocco D, Scavone G et al. (2013). The Role of Collagen Neoepitopes Generated by Oxidative Stress in the Pathogenesis of Charcot Neuroarthropathy. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Winyard⁎ PG, Nissim A, Ryan B et al. (2012). Measurement of serum autoantibodies against oxidatively modified autoantigens in human autoimmune diseases. nameOfConference


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  • Harel D, Marron A, Nissim A et al. (2012). A Software Engineering Framework for Switched Fuzzy Systems. 2012 IEEE International Conference on Fuzzy Systems


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  • Hughes C, Faurholm B, Dell'Accio F et al. (2012). TARGETING THERAPEUTICS TO ARTHRITIC JOINTS BY ANTIBODY SPECIFIC TO POST-TRANSLATIONALLY MODIFIED COLLAGEN TYPE II. nameOfConference


    QMRO: qmroHref
  • Kamperidis P, Kamalati T, Ferrari M et al. (2011). Development of a novel recombinant biotherapeutic with applications in targeted therapy of human arthritis.. nameOfConference


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  • Winyard PG, Ryan B, Eggleton P et al. (2011). Measurement and meaning of markers of reactive species of oxygen, nitrogen and sulfur in healthy human subjects and patients with inflammatory joint disease.. nameOfConference


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  • Hughes C, Faurholm B, Dell'Accio F et al. (2011). TARGETING THERAPEUTICS TO ARTHRITIC JOINTS. nameOfConference

    DOI: doi

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  • Kamperidis P, Kamalati T, Ferrari M et al. (2011). Development of a Novel Recombinant Biotherapeutic with Applications in Targeted Therapy of Human Arthritis.. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Anger J, Rogo-Gupta L, Behniwal A et al. (2011). IS "OAB-DRY" REALLY DRY?. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Eggleton P, Ryan B, Brown S et al. (2011). POST-TRANSLATIONAL MODIFICATIONS OF C1Q LEAD TO ANTIGENICITY AND BREAKDOWN OF IMMUNE TOLERANCE IN SYSTEMIC LUPUS ERYTHEMATOSUS. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Hughes C, Faurholm B, Dell'Accio F et al. (2011). Targeting therapeutics to arthritic joints. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Nissim A, DiCara D (2010). Methods for Development of Monoclonal Antibody Therapeutics. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Hendler A, Mulli TK, Hughes FJ et al. (2010). Involvement of Autoimmunity in the Pathogenesis of Aggressive Periodontitis. nameOfConference


    QMRO: qmroHref
  • Hughes C, Faurholm B, Dell'Accio F et al. (2010). Human single-chain variable fragment that specifically targets arthritic cartilage.. nameOfConference


  • Wythe SE, DiCara D, Finucane C et al. (2010). TARGETING THE INFLAMED MICROVASCULATURE IN RHEUMATOID ARTHRITIS. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Ryan B, Eggleton P, Brown S et al. (2010). Oxidative and Nitrative Modification of C1q Affects its Structure, Function and Antigenicity in Systemic Lupus Erythematosus. nameOfConference


    QMRO: qmroHref
  • Ryan B, Winyard PG, Viner N et al. (2009). OXIDATIVE MODIFICATIONS TO C1Q INCREASE THE SENSITIVITY OF AN ANTI-C1Q ELISA IN THE DIAGNOSIS OF SYSTEMIC LUPUS ERYTHEMATOSUS. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Maggiore C, Meier UC, Marta M et al. (2009). B Cells and EBV Infection in People with MS. nameOfConference

    DOI: doi

  • Nissim A, Chernajovsky Y (2008). Preface. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Maggiore C, Trillo-Pazos G, Keir G et al. (2008). Ectopic germinal centres in the brain of people with multiple sclerosis. nameOfConference

    DOI: doi

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  • Nissim A, Chernajovsky Y (2008). Historical development of monoclonal antibody therapeutics.. nameOfConference


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  • Nissim A (2008). NOVEL ANTIBODY AND USE IN DIAGNOSIS AND THERAPY OF ARTHROPATHIES. nameOfConference

    DOI: doi

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  • Ryan B, Szestakowska D, Viner N et al. (2007). Autoantibodies target oxidatively modified proteins of the C1q/calreticulin/CD91 apoptotic pathway in systemic lupus erythematosus. nameOfConference

    DOI: doi

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  • Brunton F, Lee L, Nissim A et al. (2007). Targeting osteoclasts in rheumatoid arthritis via the alpha(v)beta(3) integrin. nameOfConference

    DOI: doi

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  • Kamperidis PS, Lee L, Blades M et al. (2007). Identification of single-chain antibody fragments specific for human synovial microvascular endothelium by in vivo phage display. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Brunton F, Lee L, Nissim A et al. (2007). Modulating osteoclast function by targeting the alphavbeta3 integrin. nameOfConference

    DOI: doi

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  • Nissim A, Chernajovsky Y (2007). Therapeutic Antibodies. nameOfConference

    DOI: doi

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  • Eggleton P, Szestakowska D, Winyard PG et al. (2006). Generation of neo-antigenic epitopes recognised by autoimmune sera after the post-translational modification of human C1q by free radicals. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Winyard PG, Nissim A, Davies C et al. (2006). Nitrosative stress in the inflamed joints of rheumatoid arthritis patients. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Szestakowska D, Young P, Morse R et al. (2006). Role of oxidative protein modifications in the generation of neoantigens recognised by lupus patient sera. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Chernajovsky Y, NISSIM A, Perrett D et al. (2005). Generation of neoantigenic epitopes after posttranslational modification of type II collagen by factors present within the inflamed joint. nameOfConference


    QMRO: qmroHref
  • Nissim A, Winyard PG, Corrigall V et al. (2005). Generation of neoantigenic epitopes after posttranslational modification of type II collagen by factors present within the inflamed joint.. nameOfConference


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  • Yudkoff M, Daikhin Y, Nissim A et al. (2005). Brain amino acid requirements and toxicity: The example of leucine (vol 135, pg 1531S, 2005). nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Horyn O, Luhovyy B, Lazarow A et al. (2005). Biosynthesis of agmatine in isolated mitochondria and perfused rat liver: studies with N-15-labelled arginine. nameOfConference


    QMRO: qmroHref
  • Nissim A, Winyard P, Davis C et al. (2005). Development of human antibody fragments against collagen type II autoantigens in rheumatoid arthritis, using phage display libraries. nameOfConference

    DOI: doi

    QMRO: qmroHref
  • Inglis JJ, Nissim A, Lees DM et al. (2005). The differential contribution of tumour necrosis factor to thermal and mechanical hyperalgesia during chronic inflammation.. nameOfConference


  • Nissim A, Winyard P, Fatah R et al. (2004). Generation of neo-antigenic epitopes after the post-translational modification of type II collagen by factors present within the inflamed joint.. nameOfConference

    DOI: doi

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  • Nissim A, Gofur Y, Vessillier S et al. (2004). Methods for targeting biologicals to specific disease sites.. nameOfConference


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  • NISSIM A, Nechushtan H, Cohen-Saidon C et al. (2003). A novel strategy using single chain antibody to show the importance of Bcl-2 in mast cell survival.. nameOfConference


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  • NISSIM A, Loyter A, Piller SC et al. (2003). Antibody Fragments Selected by Phage Display Against the Nuclear Localization Signal of the HIV-1 Vpr Protein Inhibit Nuclear Import in Permeabilized and Intact Cultured Cells.. nameOfConference


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  • Yagil E, Beacham IR, Nissim A et al. (1978). Crypticity of periplasmic enzymes Involvement of protein b in the permeability of the outer membrane of Escherichia coli. nameOfConference


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  • Gargir A, Ofek I, Meron-Sudai S et al. (2002). Single chain antibodies specific for fatty acids derived from a semi-synthetic phage display library. nameOfConference


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  • Flores-Flores C, Nissim A, Shochat S et al. (2002). Development of human antibody fragments directed towards synaptic acetylcholinesterase using a semi-synthetic phage display library. nameOfConference

    DOI: doi

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  • NISSIM A, Soreq H, Flores-Flores C et al. (2002). Phage display selection and characterization of myc-tagged humanized monoclonal antibodies to synaptic acetylcholinesterase.. nameOfConference

    DOI: doi

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  • Gargir A, Ofek I, Hasty D et al. (2001). Inhibition of anti body-dependent stimulation of lipoteichoic acid-treated human monocytes and macrophages by polyglycerolphosphate-reactive peptides. nameOfConference


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  • Kjaer S, Wind T, Ravn P et al. (2001). Generation and epitope mapping of high-affinity scFv to eukaryotic elongation factor 1A by dual application of phage display. nameOfConference


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  • Vaisman N, Nissim A, Klapper LN et al. (2000). Specific inhibition of the reaction between a tumor-inhibitory antibody and the ErbB-2 receptor by a mimotope derived from a phage display library.. nameOfConference


    QMRO: qmroHref
  • Uchanska-Ziegler B, Xugang Q, Volz A et al. (2000). Human Single-Chain Fv Fragments Specific for Natural Killer Cell Receptors from Phage Display Libraries. nameOfConference


    QMRO: qmroHref
  • Uchanska-Ziegler B, Qiao X, Volz A et al. (2000). Human single-chain Fv fragments specific for natural killer cell receptors from phage display libraries.. nameOfConference


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  • Neri D, Pini A, Nissim A (publicationYear). Antibodies from Phage Display Libraries as lmmunochemical Reagents. nameOfConference


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  • Neri D, Pini A, Nissim A (1998). Antibodies from phage display libraries as immunochemical reagents.. nameOfConference


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  • Neri D, Carnemolla B, Nissim A et al. (1997). Targeting by affinity-matured recombinant antibody fragments of an angiogenesis associated fibronectin isoform. nameOfConference


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  • Neri D, Carnemolla B, Nissim A et al. (1997). Targeting by affinity-matured recombinant antibody fragments of an angiogenesis associated fibronectin isoform.. nameOfConference


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  • Evenor D, Levi-Nissim A, Afgin L et al. (1997). Regeneration of plantlets and bulblets from explants and callus of Allium aflatunense cultivars and selection from indigenous Israeli Allium ampeloprasum. nameOfConference


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  • Oren-Shamir M, Levi-Nissim A (1997). UV-light effect on the leaf pigmentation of Cotinus coggygria 'Royal Purple'. nameOfConference


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  • Nissim A, Neri D, Winter G (1996). Development and usage of semi-synthetic scFv repertoires displayed on phage. nameOfConference

    DOI: doi

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  • Harrison JL, Williams SC, Winter G et al. (1996). Screening of phage antibody libraries.. nameOfConference


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  • Nissim A, Schwarzbaum S, Eshhar Z (1995). The Use of Mouse/Human IgE Chimeras to Map the FcϵR Binding Site of IgE. nameOfConference


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  • GRIFFITHS AD, WILLIAMS SC, HARTLEY O et al. (1994). ISOLATION OF HIGH-AFFINITY HUMAN-ANTIBODIES DIRECTLY FROM LARGE SYNTHETIC REPERTOIRES. nameOfConference


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  • Nissim A, Hoogenboom HR, Tomlinson IM et al. (1994). Antibody fragments from a 'single pot' phage display library as immunochemical reagents.. nameOfConference


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  • NISSIM A, HOOGENBOOM HR, TOMLINSON IM et al. (1994). ANTIBODY FRAGMENTS FROM A SINGLE POT PHAGE DISPLAY LIBRARY AS IMMUNOCHEMICAL REAGENTS. nameOfConference

    DOI: doi

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  • Nissim A, Schwarzbaum S, Siraganian R et al. (1993). Fine specificity of the IgE interaction with the low and high affinity Fc receptor.. nameOfConference


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  • Nissim A, Eshhar Z (1992). The human mast cell receptor binding site maps to the third constant domain of immunoglobulin E.. nameOfConference


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  • Rutherford MN, Kumar A, Nissim A et al. (1991). The murine 2-5A synthetase locus: three distinct transcripts from two linked genes.. nameOfConference


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  • NISSIM A, ESHHAR Z (1991). THE MAST-CELL BINDING-SITE MAPPS TO THE 3RD CONSTANT REGION DOMAIN OF IMMUNOGLOBULIN-E. nameOfConference

    DOI: doi

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  • Nissim A, Eshhar Z (1991). Localization of the Fc epsilon RI binding site to the third constant domain of IgE.. nameOfConference


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  • Nissim A, Jouvin MH, Eshhar Z (1991). Mapping of the high affinity Fc epsilon receptor binding site to the third constant region domain of IgE.. nameOfConference


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  • Coccia EM, Romeo G, Nissim A et al. (1990). A full-length murine 2-5A synthetase cDNA transfected in NIH-3T3 cells impairs EMCV but not VSV replication.. nameOfConference


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  • Schwarzbaum S, Nissim A, Alkalay I et al. (1989). Mapping of murine IgE epitopes involved in IgE-Fc epsilon receptor interactions.. nameOfConference


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  • David S, Nissim A, Chebath J et al. (1989). 2'-5'-oligoadenylate synthetase gene expression in normal and murine sarcoma virus-transformed NIH 3T3 cells.. nameOfConference


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  • NISSIM A, COCCIA E, COHEN B et al. (1987). 3 DIFFERENT 2'-5'OLIGO A SYNTHETASE GENES ARE ACTIVE IN MOUSE CELLS AND MULTIPLE FORMS OF ENZYMES ARE INDUCED BY INTERFERONS. nameOfConference

    DOI: doi

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  • CHITAYAT SD, NISSIM A, COCCIA C et al. (1987). 2-5A SYNTHETASE GENE-EXPRESSION IN NORMAL AND MSV-TRANSFORMED NIH/3T3 CELLS. nameOfConference

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Collaborators

Internal

  • Costantinos Pitzalis (WHRI)
  • Michele Bombardieri (WHRI)
  • Francesco Dell'Accio (WHRI)
  • Gavin Giovannoni (Blizard)
  • David Lesly (Blizard)
  • John Marshal (BCI)
  • Helena Azevedo (School of Engineering)
  • James Garnett (School of Biological and Chemical Sciences)
  • Roberto Buccafusca (School of Biological and Chemical Sciences)

External

  • Paul G Winyard (Exeter)
  • Pozzilli Paolo (Rome)
  • Iain B McInnes, Carl S Goodyear (Glasgow)
  • Frederique Ponchel, Philip G Conaghan, Paul Emery (Leeds)
  • Tonia Vincent (Kennedy)
  • Maria Infantino and Maurizio Benucci (Florence)
  • Howard Amital (Israel)
  • Zelig Eshhar (Israel)
  • Industrial collaborators from Europe, Israel and US

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